Status of SARS-CoV-2 detection systems (RT-PCR)
enabled by data from
Update: Sun Jan 31 07:00:30 2021 (UTC+2)
Author: Olivier Friard - Department of Life Sciences and Systems Biology - University of Turin - Italy
We gratefully acknowledge the Authors of the Originating and Submitting laboratories of the genetic sequence and
metadata shared via GISAID, on which this research is based.
The isolates collection dates are in YYYY-MM-DD format (ISO 8601).
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Number of isolates sequences available: 15
When the target sequence of amplification systems is available the primers and probe are aligned against the target sequence using the Smith-Waterman local sequence alignment algorithm and the alignments are classified in 3 categories:
Full match - All primers and probe have a 100% identity with the target sequence. In case of a 2 probes system (RdRP) at least one probe must have a 100% identity.
The identities of forward/reverse primers AND probe are greater than or equal to 80% AND the length of the amplification product is comprised between the reference length plus/minus 8 bp.
The identity of one primer OR the probe are less than 80% OR the length of the amplification product is NOT comprised between reference length plus/minus 8 bp.
If the target sequence is not available the The sequence is not available
warning is shown.
Please note that the reverse primer sequence is shown reverted-complemented in the alignments.
Move the mouse cursor over elements to see the details.
|Virus name / Accession ID||Region||Collection date||2019-nCoV_N1||2019-nCoV_N2||2019-nCoV_N3||E-Sarbeco||RdRP|