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Status of SARS-CoV-2 detection systems (RT-PCR)
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Update: Sun Oct 25 07:18:47 2020 (UTC+2)

Author: Olivier Friard - Department of Life Sciences and Systems Biology - University of Turin - Italy

We gratefully acknowledge the Authors of the Originating and Submitting laboratories of the genetic sequence and metadata shared via GISAID, on which this research is based.
The isolates collection dates are in YYYY-MM-DD format (ISO 8601).

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Estonia

Number of isolates sequences available: 20

Method

When the target sequence of amplification systems is available the primers and probe are aligned against the target sequence using the Smith-Waterman local sequence alignment algorithm and the alignments are classified in 3 categories:
             Full match - All primers and probe have a 100% identity with the target sequence. In case of a 2 probes system (RdRP) at least one probe must have a 100% identity.
             The identities of forward/reverse primers AND probe are greater than or equal to 80% AND the length of the amplification product is comprised between the reference length plus/minus 8 bp.
             The identity of one primer OR the probe are less than 80% OR the length of the amplification product is NOT comprised between reference length plus/minus 8 bp.

If the target sequence is not available the The sequence is not available warning is shown.
Please note that the reverse primer sequence is shown reverted-complemented in the alignments.

Results

Move the mouse cursor over elements to see the details.

Virus name / Accession IDRegionCollection date2019-nCoV_N12019-nCoV_N22019-nCoV_N3E-SarbecoRdRP
Estonia/ChVir1982/2020 EPI_ISL_4200652020-03
Estonia/ChVir1983/2020 EPI_ISL_4200662020-03
Estonia/ChVir1985/2020 EPI_ISL_4200672020-03
Estonia/ChVir2148/2020 EPI_ISL_4205402020-03
EPI_ISL_450498
EPI_ISL_457719
EPI_ISL_457720
EPI_ISL_457722
EPI_ISL_457723
EPI_ISL_457725
EPI_ISL_457727
EPI_ISL_457729
EPI_ISL_457731
EPI_ISL_457734
EPI_ISL_457735
EPI_ISL_457737
EPI_ISL_457738
EPI_ISL_457739
EPI_ISL_457740
EPI_ISL_516627

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